Activities Year 2024

The Department of Biochemistry is pleased to share that our team successfully participated in the the 52th Annual Phramongkutklao Hospital Academic Conference, focusing on innovative techniques for protein extraction from FFPE breast cancer tissues. The poster, titled "Optimizing Protein Extraction from Formalin-Fixed Paraffin-Embedded (FFPE) Breast Cancer Tissue: An Efficient Procedure and the Impact of pH in Extraction Buffers".

Team Members:

• Montalee Theeraapisakkun – Department of Biochemistry, Phramongkutklao College of Medicine

• Tidatip Pimphiw – Department of Medical Technology, Faculty of Allied Health Sciences, Pathumthani University

• Panipak Khakhanmalee, Sirintorn Somjai, Thanakon Pinasa, Pasra Arnutti, Unchalee Visawapoka – Department of Medical Technology, Faculty of Allied Health Sciences, Pathumthani University

• Natcha Lorsuwannarat – Department of Anatomy, Phramongkutklao College of Medicine

🔍 Research Highlights:

• Developed cost-effective methods for protein extraction using Tris HCl buffers at pH 7 and 9.

• Protocol 2 (90°C for 120 min) yielded the highest protein concentrations, outperforming commercial kits.

• SDS-PAGE analysis confirmed protein quality comparable to standard methods.

Conclusion:
This study provides an efficient and affordable alternative for protein extraction from FFPE tissues, enabling broader access to research tools for disease progression, prognosis, and treatment strategies.

We thank all contributors and participants for their support and look forward to continuing advancements in molecular biology and cancer research!